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Human renal cortex

Biopsy tissue 1

DOI

This a small four channel confocal 3D volume. The four fluorescence channels are, in order, DAPI, Phalloidin-A488, AQP2 and AQP1. The volume contains a crosssection of a glomerulus, vessels and a field of proximal tubules, putative distal convoluted tubules and collecting duct.

Image file

   
Filename Kidney_Cortex_Human.tif
Size 84.1 MB
Modality Confocal
Dimensions (pixels) 512x512x40
Dimensions (microns) 635.9x635.9x40
Channels 4
Reference Woloshuk et al, Winfree et al

VTEA files

Use Filename
Objects XXXXXX.obx
Image XXXXX.tif
Image processing XXXXX.prc
Documentation XXXX.txt
Reference Winfree et al

Biopsy tissue 2

DOI

This is an image volume of a kidney biopsy collected with a cyclic multiplexed immunofluorescense approach, CODEX.

Image file

   
Filename Kidney_Cortex_Human_CODEX.tif
Size 2.3 GB
Modality Widefield/CODEX
Dimensions (pixels) 6592x3760x1
Dimensions (microns) 2490x1420
Channels 44
Reference Winfree et al

VTEA files

Use Filename
Objects XXXXXX.obx
Image XXXXX.tif
Image processing XXXXX.prc
Documentation XXXX.txt
Reference Winfree et al

Nephrectomy tissue 1

DOI

This a large four channel confocal 3D volume orginally used to extract labeled nuclei in this publication. The four fluorescence channels are, in order, DAPI, XXXX, YYYY and ZZZZ. The volume contains a broad crosssection of the cortex and outer medulla with glomeruli, vessels, tubules and immune cells.

Image file

   
Filename Human_Kidney_Cortex_Mesoscale.tif
Size 14.4 GB
Modality Confocal
Dimensions (pixels)  
Dimensions (microns)  
Channels 4
Reference Woloshuk et al, Winfree et al

VTEA files

Use Filename
Objects XXXXXX.obx
Image XXXXX.tif
Image processing XXXXX.prc
Documentation XXXX.txt
Reference Winfree et al

Nephrectomy tissue 2

DOI

This nephrectomy tissue was stained and imaged using a protocol developed for the Kidney Precision Medicine Project and published here. The eight fluorescence channels are, in order, Phalloidin-A488, THP, AQP1, MPO, CD68, CD3, Siglec-8, and DAPI.

Image file

   
Filename Kidney_Cortex_Human_Spectral_1.tif
Size 1.8 GB
Modality Confocal
Dimensions (pixels) 1704x3534x19
Dimensions (microns) 921x1911x19
Channels 8
Reference Woloshuk et al, Winfree et al

VTEA files

Use Filename
Objects XXXXXX.obx
Image XXXXX.tif
Image processing XXXXX.prc
Documentation XXXX.txt
Reference Winfree et al